SYNBIOS® SYNTHETIC PROTEIN-FREE MEDIA ELIMINATES DISEASE TRANSMISSION

  • SIGNATURE SPECIALTY SYNTHETIC  PROTEIN-FREE MEDIA
  • CONVENTIONAL PROTEIN-SUPPLEMENTED MEDIA

Human day 2 embryo generated in a SYNTHETIC PROTEIN-FREE (ART-7b) MEDIUM (fertilization up to day 2). Embryo transfer (n=3) was performed on day 2. The ART-7b medium has been phased out. This embryo is now a young man in his early twenties, one of a set of triplets (all boys). This was the first report of the successful generation of human embryos in a completely synthetic medium devoid of donor serum proteins. (Proc. XVI Ann. Sci Meeting Fertil Soc Australia, 2-4 Nov, 1997, Adelaide, Australia, p.34; Ali et al., Human Reprod 15:145, 2000).

Human day 3 embryo generated in PFM-11 SYNTHETIC PROTEIN-FREE MEDIUM from fertilization up to day 3.  The ART-7b and PFM-11 media have been phased out. The SYNBIOS®embyro culture and handling media is an improved formulation over its predecessors, the ART-7b and PFM-11 synthetic protein-free media.

A human blastocyst that developed in SYNBIOS® SYNTHETIC EMBRYO CULTURE MEDIUM. Image was captured using the embryoscope. The workers noted that the performance of the SYNBIOS® SYNTHETIC EMBRYO CULTURE MEDIUM was comparable to contemporary conventional protein-containing medium. Courtesy: Yousef AlHelou, April 2020, Fakih IVF, United Arab Emirates.

A hatching human blastocyst that developed in SYNBIOS® SYNTHETIC EMBRYO CULTURE MEDIUM. Image was captured using the embryoscope. The hatching occurred naturally. The workers noted that the performance of the  SYNBIOS® SYNTHETIC EMBRYO CULTURE MEDIUM was comparable to contemporary conventional protein-containing medium.
Courtesy: Yousef AlHelou, April 2020, Fakih IVF, United Arab Emirates.

A hatching human blastocyst that developed in SYNBIOS® SYNTHETIC EMBRYO CULTURE MEDIUM. Image was captured using the embryoscope. The hatching occurred following laser assisted hatching. The workers perform routine laser assisted hatching on day 3 for 80% of their patients because their treatment SOP is to perform biopsy on day 5-7.
Courtesy: Yousef AlHelou, April 2020, Fakih IVF, United Arab Emirates.

A hatching human blastocyst that developed in SYNBIOS® SYNTHETIC EMBRYO CULTURE MEDIUM. Image was captured using the embryoscope. The hatching occurred following laser assisted hatching on day 3.

Courtesy: Yousef AlHelou, April 2020, Fakih IVF, United Arab Emirates.

A hatching human day 6 blastocyst (picture taken in the morning) that developed in culture after vitrification on day 2 at the 4-cell stage with the (SYNBIOS®) VS14 VITRIFICATION SOLUTION. This was the first report of successful cryopreservation of the human embryo by the ultra-rapid cryopreservation technique of vitrification. VS14 vitrification almost always resulted in complete survival post warming and rehydration with no appreciable loss of viability both in vitro and in vivo in mammalian embryos, viz: mouse, sheep and human. (Proceedings of the 1994 Annual Scientific Meeting of the Fertility Society of Australia, Brisbane, 4-7 October 1994;  Ali et al., 1995. Med. Sci. Res.(UK) 23:539-540, 1995).

A completely hatched human embryo. It was vitrified on day 2. This blastocyst is the same  day 6 hatching blastocyst seen in image 7 above (picture was taken in late afternoon).  It hatched out completely by late afternoon of the same day.(Proceedings of the 1994 Annual Scientific Meeting of the Fertility Society of Australia, Brisbane, 4-7 October 1994;  Ali et al., 1995. Med. Sci. Res.(UK) 23:539-540, 1995).

Late day 5 hatching human blastocyst that developed from a vitrified day 3 parthenogenetic 1PN embryo. The vitrification solution used was the SYNBIOS®VS34, a chemically defined and completely synthetic protein-free vitrification solution.

Day 6 Sheep compacted morula immediately after exposure to SYNBIOS® vitrification solution. When exposed to vitrification solution it shrunk immediately assuming the shape of a deflated ball due to rapid dehydration. This ultra-rapid vitrification technique of cryopreservation resulted in in vitro and in vivo viability comparable to fresh untreated sheep day 6 embryos. Ali and Shelton, 1992. Proceedings of the Annual Conference of the Australian Society for Reproductive Biology, Adelaide, Australia. (Ali and Shelton, J Reprod Fertil. 99:65-70, 1993).

Day 50 sheep fetus. This fetus developed from a vitrified day 6 sheep embryo following surrogate embryo transfer. It proceeded to normal term delivery and lived for about a decade. It was named “Arthur”. A number of live births were obtained in the sheep from vitrified embryos during the same period. Live births obtained for vitrified sheep embryos were comparable to that of fresh embryos. No teratogenicity was noted in sheep obtained from vitrified embryos. (Ali and Shelton, J Reprod Fertil. 99:65-70, 1993). Courtesy: Dr Peter McCullagh and Dr Jim Shelton, Div of Clin Sci, John Curtin School of Medical Research, The Australian National University.

Mouse day 18 fetuses that developed from VS14-vitrified day 4 embryos. The survival following vitrification and rehydration and, in vitro and in vivo viability of vitrified embryos was comparable to untreated fresh day 4 mouse embryosTeratogenicity was not noted in mouse fetuses obtained from vitrified day 4 sheep embryos. (Ali and Shelton , J. Reprod. Fertil.,98:459-465,1993)

A litter of mice derived from vitrified Swiss Outbred mouse embryos. This proved vitrified mouse embryos are viable and can result in normal young. The C57BL black mouse in the picture is the surrogate. Teratogenicity was not noted in both fetuses as well as live-born litters generated from vitrified mouse embryos. (Ali and Shelton,J. Reprod. Fertil.,98:459-465,1993).

Mice derived from vitrified embryos could reproduce and give birth to normal young. Both parents of this litter were vitrified on day 4. No teratogenicity was noted in the litters generated by parents that were derived from vitrified embryos. (Ali and Shelton,J. Reprod. Fertil.,98:459-465,1993)

Future possibilities. Day 3 human 8-cell embryo generated in SYNBIOS®SYNTHETIC PROTEIN-FREE medium in an atmosphere of air without CO2 incubation. This incubation system has done away with the need for both donor proteins as well carbon dioxide incubation gas for culture and growth of embryos in vitro.

Mouse blastocysts derived from day 1 zygotes in media containing BSA protein, cultured in an atmosphere of air without CO2 incubation gas. The proportion of blastocyst that developed in this culture system was statistically similar to the control cultured under similar conditions but in an atmosphere of 5% CO2. (Ali J, WK Whitten and Shelton JN. (1993). Human Reprod. 7:1110 – 1114)

Human cumulus cells attached to the base of the dish growing in the SYNBIOS® SYNTHETIC PROTEIN-FREE EMBRYO CULTURE medium. The synthetic medium can support the growth of human cumulus, granulosa and stem cells. It is likely it may support the development and growth of other cells as well.

Human cumulus cells growing in clumps in the SYNBIOS® SYNTHETIC PROTEIN-FREE EMBRYO CULTURE MEDIUM.